ER Alpha / Estrogen Receptor Antibody (aa582-595, clone C0542)
Catalogue Number: LS-C23274-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.09% sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | ESR1, Era, ERalpha, Estrogen receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha |
| Shipping Condition: | RT |
| Unit(s): | 200 ug, 50 ug |
| Host name: | Mouse |
| Clone: | C0542 |
| Isotype: | IgG1 |
| Immunogen: | A 14 residue C-Terminus synthetic peptide, (C)YYITGEAEGFPATV based on the human estrogen receptor (aa 582-595) alpha protein sequence with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%); Gorilla, Monkey (93%); Bat, Bovine, Horse (86%). |
| Application: | ICC, IP, WB, IHC, EMSA |
Additional Text
Concentration
0.5 mg/ml
Gene ID
2099
Gene Name
ESR1
Purification
Protein G purified
Antigen Type
Synthetic Peptide, KLH Conjugated
Short Description
Estrogen Receptor antibody LS-C23274 is an unconjugated mouse monoclonal antibody to human Estrogen Receptor (ER Alpha) (aa582-595). Validated for GS, ICC, IHC, IP and WB.
Storage Note
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
Suitable for use in Western Blot, Gel Mobility Shift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (ECL): 2 ug/ml. Gel Mobility Shift Assay: 20 ug/reaction. Immunoprecipitation: 20 ug/100 ul beads. We recommend that protein A Sepharose be used as immunoabsorbant with a bridging rabbit anti-mouse IgG to ensure efficient binding of the antibody.
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