Histone H4 Antibody (Acetyl-Lys12)

Catalogue Number: LS-C24398-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	0.05% Sodium azide
Type:	Polyclonal Primary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	100 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Ovalbumin-conjugated, synthetic peptide containing the sequence (. LG[acK]GG.) in which acK corresponds to acetyl-lysine 12 of yeast Histone H4.
Application:	IP, WB, FA

Overview

Additional Text

Antigen Type

Synthetic Peptide

Modification

Acetylated, Lys12

Short Description

Histone H4 antibody LS-C24398 is an unconjugated rabbit polyclonal antibody to Histone H4 (Acetyl-Lys12) from human and yeast. Validated for Func, IP and WB.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

Suitable for use in Peptide Inhibition, Peptide Dot Blot, Western Blot and Immunoprecipitation. Peptide Inhibition: 10 uM of a histone H4 peptide containing acetyl-lysine 12 abolished detection of histone H4 by anti-acetyl-Histone H4 (Lys12) in immunoblot analysis of HeLa acid extracts. Little to no signal reduction was observed with 10 uM of histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16. Peptide Dot Blot: A 1:1000 detects serial dilutions (25, 5, 1 and 0.2uM) of a histone H4 peptide containing acetyl-lysine 12 spotted onto PVDF, and not histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16. Western Blot: 1:3000 to 1:10000 detects acetyl-Histone H4 (Lys12) in acid extracted proteins from HeLa cells. The antibody did not detect unacetylated recombinant Histone H4. Immunoprecipitation: 2-6 ul immunoprecipitated the Ime2 promoter from wild type yeast chromatin and not from mutant yeast chromatin containing a histone H4 K12R mutation.

Price

£1,178.00

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