RPS6KB1 / P70S6K / S6K Antibody (aa26-43)

Catalogue Number: LS-C25941-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	0.05% Sodium azide
Type:	Polyclonal Primary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	200 ug
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Synthetic peptide corresponding to residues 26-43 of rat S6 Kinase. The immunizing sequence is identical in mouse and human.
Application:	IP, WB, FA

Overview

Additional Text

Gene ID

6198

Gene Name

RPS6KB1

Antigen Type

Synthetic Peptide

Purification

Protein A purified

Specificity

RPS6KB1, 70 kDa S6 kinase, p70 S6KA, p70-alpha, p70-S6K, p70 S6 kinase alpha, p70s6 kinase, S6K-beta-1, STK14A, p70 ribosomal S6 kinase, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70-S6K 1, p70S6K, p70S6K1, PS6K, Ribosomal protein S6 kinase I, S6K, S6K1, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha

Short Description

S6K antibody LS-C25941 is an unconjugated rabbit polyclonal antibody to S6K (RPS6KB1 / P70S6K) (aa26-43) from rat. It is reactive with human, mouse and rat. Validated for Func, IP and WB.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 0.5-2.0 ug/ml detects p70 S6 kinase in RIPA lysates from HEK293, PC-12, and A431 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (2 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitates p70 S6 kinase from 500 ug of HEK293 RIPA lysate. Immunoprecipitation Kinase Assay: 5 ug immunoprecipitates active p70 S6 kinase from IGF-1 stimulated (15nM) human HEK293 cell lysate containing 1 uM microcystin. The assay was performed using a S6 Kinase Assay Kit, which uses specific substrate peptide as a kinase substrate and contains a separate inhibitor cocktail which blocks the activity of other serine/threonine kinases such as protein kinase A, protein kinase C, and calmodulin dependent kinases.

Price

£1,027.00

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