Donkey Anti-Goat IgG Secondary Antibody (HRP)
Catalogue Number: LS-C346458-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Physical state: | Lyophilized |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Donkey |
| Clone: | |
| Isotype: | |
| Immunogen: | Purified normal IgG isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Application: | ELISA, ICC, ID, IHC-P, DB, IHC |
Additional Text
Antigen Type
Purified protein
Purification
Purified
Short Description
Goat IgG antibody LS-C346458 is an HRP-conjugated donkey polyclonal antibody. Validated for DB, ELISA, ICC, ID and IHC.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of goat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable no-precipitating antibody-binding assays between 1:2000 and 1:15000.
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