IgD Fc Antibody
Catalogue Number: LS-C346574-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Physical state: | Lyophilized |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 10 mg |
| Host name: | Goat |
| Clone: | |
| Isotype: | |
| Immunogen: | Purified polyclonal and monoclonal IgD isolated from BALB/C and C57BL mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Application: | ELISA, IF, DB |
Additional Text
Antigen Type
Purified protein
Purification
Protein G purified
Short Description
IgD Fc antibody LS-C346574 is an unconjugated goat polyclonal antibody to mouse IgD Fc. Validated for DB, ELISA and IF.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
The reactivity of the antiserum is directed to the Fc subunit of the IgD molecule which expresses strict isotypic (class) specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against serum of mice belonging to different allotypic groups, a single precipitin line is obtained which show a reaction of identity with the precipitin lines obtained with the purified IgD of BALB/C and C57BL origin used as immunogens. It does not react with IgG, including all subclasses, IgM and IgG/Fab or any non- Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also reacts with membrane-bound IgD in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.
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