Goat Anti-Rat IgG2b Secondary Antibody

Catalogue Number: LS-C346728-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	No preservative
Physical state:	Lyophilized
Type:	Polyclonal Secondary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	10 mg
Host name:	Goat
Clone:
Isotype:
Immunogen:	Pools of purified homogenous IgG2b isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Application:	ELISA, IF, DB

Overview

Additional Text

Antigen Type

Purified protein

Purification

Purified

Short Description

Rat IgG2b antibody LS-C346728 is an unconjugated goat polyclonal antibody. Validated for DB, ELISA and IF.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

The reactivity of the antiserum is directed to the subclass IgG2b as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to prepare conjugates of the user's own choice. to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.

Price

£545.00

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