Rabbit Anti-Goat IgM Secondary Antibody (HRP)
Catalogue Number: LS-C346885-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Physical state: | Lyophilized |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Purified normal IgM isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Application: | ELISA, ICC, ID, IHC-P, DB, IHC |
Additional Text
Antigen Type
Purified protein
Purification
Purified
Short Description
Goat IgM antibody LS-C346885 is an HRP-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, ICC, ID and IHC.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgM molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in sheep serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:2000 and 1:10000.
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