IgG Fab Antibody (HRP)

Catalogue Number: LS-C347008-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	No preservative
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	1 ml
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	Purified normal Fab of polyclonal rhesus monkey IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
Application:	ELISA, ICC, ID, IHC-P, DB, IHC

Overview

Additional Text

Antigen Type

Purified protein

Purification

Purified

Short Description

IgG Fab antibody LS-C347008 is an HRP-conjugated rabbit polyclonal antibody to monkey IgG Fab. Validated for DB, ELISA, ICC, ID and IHC.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits, and with free light chains of kappa and lambda type. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled monkey serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab. Direct staining of fixed cell and tissue substrates. to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:5000 and 1:10000.

Price

£647.00

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