Lambda Light Chain Antibody (HRP)

Catalogue Number: LS-C347114-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:No preservative
Physical state:Lyophilized
Type:Polyclonal Primary Antibody - Conjugated
Shipping Condition:RT
Unit(s): 1 ml
Host name: Sheep
Clone:
Isotype:
Immunogen: A pool of purified Bence Jones lambda proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
Application: ELISA, ICC, IHC-P, DB, IHC

Additional Text

Antigen Type

Purified protein

Purification

Purified

Short Description

Lambda Light Chain antibody LS-C347114 is an HRP-conjugated sheep polyclonal antibody to mouse Lambda Light Chain. Validated for DB, ELISA, ICC and IHC.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda chain. A reaction is obtained with intact polyclonal or monoclonal immunoglobulins of the lambda type, as well as free lambda light chains. To identify light chain type in enzyme-immunocytochemical and immunohistochemical staining of immunoglobulins and to demonstrate circulating monoclonal immunoglobulins and Bence Jones proteins of the lambda type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:200. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.