Goat Anti-Human IgM Fc Secondary Antibody

Catalogue Number: LS-C347170-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:No preservative
Physical state:Lyophilized
Type:Polyclonal Secondary Antibody - Unconjugated
Shipping Condition:RT
Unit(s): 10 mg
Host name: Goat
Clone:
Isotype:
Immunogen: Purified homogenous IgM isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Application: ELISA, IF, DB

Additional Text

Antigen Type

Purified protein

Purification

Purified

Short Description

Human IgM Fc antibody LS-C347170 is an unconjugated goat polyclonal antibody. Validated for DB, ELISA and IF.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with IgG, IgA and IgG/Fab or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000.