Pig Anti-Mouse IgG Secondary Antibody

Catalogue Number: LS-C347211-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:No preservative
Physical state:Lyophilized
Type:Polyclonal Secondary Antibody - Unconjugated
Shipping Condition:RT
Unit(s): 10 mg
Host name: Pig
Clone:
Isotype:
Immunogen: Purified normal IgG isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Application: ELISA, IF, DB

Additional Text

Antigen Type

Purified protein

Purification

Purified

Short Description

Mouse IgG antibody LS-C347211 is an unconjugated pig polyclonal antibody. Validated for DB, ELISA and IF.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.