Complement C3c Antibody
Catalogue Number: LS-C347257-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Physical state: | Lyophilized |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Goat |
| Clone: | |
| Isotype: | |
| Immunogen: | C3c purified from pooled normal guinea pig serum by precipitation techniques, followed by chromatographical methods. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Application: | ID |
Additional Text
Antigen Type
Purified protein
Short Description
Complement C3c antibody LS-C347257 is an unconjugated goat polyclonal antibody to guinea pig Complement C3c. Validated for ID.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of mouse serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
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