Goat Anti-Human IgG Fab Secondary Antibody

Catalogue Number: LS-C347274-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	No preservative
Physical state:	Lyophilized
Type:	Polyclonal Secondary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	10 mg
Host name:	Goat
Clone:
Isotype:
Immunogen:	Purified Fab from normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Application:	ELISA, IF, DB

Overview

Additional Text

Antigen Type

Purified protein

Purification

Purified

Short Description

Human IgG Fab antibody LS-C347274 is an unconjugated goat polyclonal antibody. Validated for DB, ELISA and IF.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. For indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.

Price

£417.00

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