Ig Kappa Light Chain Antibody (Biotin)
Catalogue Number: LS-C347301-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Physical state: | Lyophilized |
| Type: | Polyclonal Secondary Antibody - Conjugated |
| Alias: | IgK, Ig Kappa |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Goat |
| Clone: | |
| Isotype: | |
| Immunogen: | A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Application: | ELISA, ICC, IHC-P, DB, IHC |
Additional Text
Antigen Type
Purified protein
Purification
Purified
Short Description
Ig Kappa Light Chain antibody LS-C347301 is a biotin-conjugated goat polyclonal antibody to mouse Ig Kappa Light Chain. Validated for DB, ELISA, ICC and IHC.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa chain. A reaction is obtained with intact polyclonal or monoclonal immunoglobulins of the kappa type, as well as free kappa light chains. To identify light chain type in immunocytochemical and immunohistochemical staining of immunoglobulins and to demonstrate circulating monoclonal immunoglobulins and Bence Jones proteins of the kappa type in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:4000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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