Mouse Anti-Human IgG1 Fc Secondary Antibody (clone NI 132 (HP 6186), Biotin)

Catalogue Number: LS-C347427-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	No preservative
Physical state:	Lyophilized
Type:	Monoclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	0.2 mg
Host name:	Mouse
Clone:	NI 132 (HP 6186)
Isotype:	IgG1
Immunogen:	Highly purified monoclonal IgG1 isolated from pooled human serum.
Application:	ELISA, ICC, IHC-P, DB, IHC

Overview

Additional Text

Antigen Type

Purified protein

Purification

Purified

Short Description

Human IgG1 Fc antibody LS-C347427 is a biotin-conjugated mouse monoclonal antibody. Validated for DB, ELISA, ICC and IHC.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Monoclonal

Application Notes

The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40. in ELISA from 1:100 to 1:2000. in Western blotting from 1:200 to 1:4000. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Price

£744.00

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