Mouse Anti-Human IgM Secondary Antibody (clone NI 179, HRP)
Catalogue Number: LS-C347438-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Physical state: | Lyophilized |
| Type: | Monoclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 0.2 mg |
| Host name: | Mouse |
| Clone: | NI 179 |
| Isotype: | IgG1 |
| Immunogen: | Highly purified monoclonal IgM isolated from pooled human serum. |
| Application: | ELISA, ICC, IHC-P, DB, IHC |
Additional Text
Antigen Type
Purified protein
Purification
Purified
Short Description
Human IgM antibody LS-C347438 is an HRP-conjugated mouse monoclonal antibody. Validated for DB, ELISA, ICC and IHC.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgM, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:250 upwards. in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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