CDH6 / K Cadherin Antibody (Extracellular Domain)

Catalogue Number: LS-C35232-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	CDH6, K Cadherin, K-cadherin, CAD6, Cadherin-6, Fetal kidney cadherin, KCAD, Kidney cadherin
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:	NS0-derived rhCadherin-6 extracellular domain.
Application:	ELISA, FC, ICC, WB

Overview

Additional Text

Gene Name

CDH6

Gene ID

1004

Purification

Affinity Purified

Short Description

K Cadherin antibody LS-C35232 is an unconjugated sheep polyclonal antibody to human K Cadherin (CDH6) (Extracellular Domain). Validated for ELISA, Flow, ICC and WB.

Antibody Clonality

Polyclonal

Storage Note

Short term: -20°C; Long term: -20°C.

Application Notes

Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Western Blot: 0.1 - 0.2 ug/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/mL to detect human Cadherin-6 in MG63 human osteosarcoma cells cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Flow Cytometry: 50 ug/ml Add 10 ul of the diluted solution to 1-2.5x10^5 MG63 human osteosarcoma cells. This antibody has been tested on MG63 human osteosarcoma cells. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit for rhCadherin-6 is approximately 1 ng/well.

Price

£1,170.00

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