CD58 Antibody (Biotin)

Catalogue Number: LS-C35694-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	0.05% Sodium azide
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 molecule
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Mouse
Clone:
Isotype:	IgG1
Immunogen:	Human cytotoxic T cells.
Application:	FC, IP, IHC-Fr, IHC, Cytox

Overview

Additional Text

Gene ID

965

Purification

Protein A purified

Gene Name

CD58

Short Description

CD58 antibody LS-C35694 is a biotin-conjugated mouse monoclonal antibody to human CD58. Validated for Ctox, Flow, IHC and IP.

Antibody Clonality

Monoclonal

Application Notes

Antibody will inhibit HLA-DR mediated T cell cytotoxicity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Immunohistochemistry: Frozen. Flow Cytometry: 5x10^5 cultured human Nalm-6 cells were washed and incubated 45 minutes on ice with 80 ml of anti-CD58/Biotin at 10 mg/ml. Cells were washed twice and incubated with 2? reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94% blocked when pre incubated 10 minutes with 20 ml of 0.5 mg/ml anti-CD58 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Price

£779.00

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