PD-L2 / PDCD1LG2 / CD273 Antibody (clone ANC8D12, Biotin)

Catalogue Number: LS-C357632-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:0.04% Sodium azide
Type:Monoclonal Primary Antibody - Conjugated
Alias:PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2
Shipping Condition:RT
Unit(s): 100 ug
Host name: Mouse
Clone: ANC8D12
Isotype: IgG2a, kappa
Immunogen: Recombinant human CD273(B7-DC).
Application: ELISA, FC, Blk

Additional Text

Concentration

0.5 mg/ml

Gene ID

80380

Gene Name

PDCD1LG2

Antigen Type

Recombinant Protein

Purification

Protein A purified

Short Description

CD273 antibody LS-C357632 is a biotin-conjugated mouse monoclonal antibody to human CD273 (PD-L2 / PDCD1LG2). Validated for Block, ELISA and Flow.

Antibody Clonality

Monoclonal

Storage Note

Store at 4°C, avoid freeze-thaw cycles.

Application Notes

Human monocyte derived Dendritic cells were prepared in 8 day culture. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of antihuman CD273/Biotin at concentration of 10 ug/ml. They were then washed twice and incubated with Streptavidin/R-PE, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to isotype control Mouse IgG2a/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.