CD8A / CD8 Alpha Antibody (clone 53-6.72, APC)
Catalogue Number: LS-C357658-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.04% Sodium azide |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD8A, CD8a molecule, CD8a antigen, OKT8 T-cell antigen, Leu2 T-lymphocyte antigen, T8 T-cell antigen, T cell co-receptor, p32, CD8, Leu2, T-cell antigen Leu2 |
| Shipping Condition: | RT |
| Unit(s): | 120 tests |
| Host name: | Rat |
| Clone: | 53-6.72 |
| Isotype: | IgG2a, kappa |
| Immunogen: | Mouse thymocytes/splenocytes. |
| Application: | ELISA, FC |
Additional Text
Gene ID
925
Antigen Type
Cells
Purification
Protein G purified
Gene Name
CD8A
Short Description
CD8 Alpha antibody LS-C357658 is an APC-conjugated rat monoclonal antibody to mouse CD8 Alpha (CD8A). Validated for ELISA and Flow.
Antibody Clonality
Monoclonal
Storage Note
Store at 4°C. Do not freeze. Protect from light.
Application Notes
Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-Mouse CD8/APC at a 1:50 dilution (2 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 9% sub population of the cells stained positive with a mean shift of 2.59 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD8 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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