CD3E Antibody (clone 145-2C11, FITC)

Catalogue Number: LS-C357689-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	0.04% Sodium azide
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon, CD3
Shipping Condition:	RT
Unit(s):	120 tests
Host name:	Hamster
Clone:	145-2c11
Isotype:	IgG
Immunogen:	Mouse BM10-37 T cell membrane prep.
Application:	ELISA, FC, IP, WB

Overview

Additional Text

Gene ID

916

Antigen Type

Membrane preparation

Purification

Protein G purified

Gene Name

CD3E

Short Description

CD3E antibody LS-C357689 is an FITC-conjugated hamster monoclonal antibody to mouse CD3E. Validated for ELISA, Flow, IP and WB.

Antibody Clonality

Monoclonal

Storage Note

Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.

Application Notes

Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-mouse CD3/FITC at a 1:50 dilution (5 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 35% sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Price

£555.00

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