CD4 Antibody (clone QS4120, PE, Cy7)
Catalogue Number: LS-C357690-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.04% Sodium azide |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 molecule |
| Shipping Condition: | RT |
| Unit(s): | 120 tests |
| Host name: | Mouse |
| Clone: | QS4120 |
| Isotype: | IgG1 |
| Immunogen: | CD4+ transfectant/human CEM |
| Application: | ELISA, FC, Blk |
Additional Text
Gene ID
920
Antigen Type
Cells
Purification
Protein A purified
Gene Name
CD4
Short Description
CD4 antibody LS-C357690 is a PE, Cy7-conjugated mouse monoclonal antibody to human CD4. Validated for Block, ELISA and Flow.
Antibody Clonality
Monoclonal
Storage Note
Store at 4°C. Do not freeze. Protect from light.
Application Notes
Reagent was tested for binding to ficoll prepared human peripheral blood mononuclear cells (PBMC) in FACS. Five x 105 PBMC per tube were washed and pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of antihuman CD4/PE-Cy7 at a 1:50 dilution (5 ug/ml). Cells were then washed three times, fixed and analyzed by FACS using a lymphoid gate. A 41% sub population of the cells stained positive with a mean shift of 2.0 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-human CD4 antibody. ELISA: Use for capture. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
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