CD3E Antibody (clone 145-2C11)
Catalogue Number: LS-C357691-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | Gentamicin |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon, CD3 |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Hamster |
| Clone: | 145-2c11 |
| Isotype: | IgG |
| Immunogen: | Mouse BM10-37 T cell membrane prep. |
| Application: | ELISA, FC, IP, WB |
Additional Text
Concentration
0.5 mg/ml
Gene ID
916
Antigen Type
Membrane preparation
Purification
Protein G purified
Gene Name
CD3E
Short Description
CD3E antibody LS-C357691 is an unconjugated hamster monoclonal antibody to mouse CD3E. Validated for ELISA, Flow, IP and WB.
Antibody Clonality
Monoclonal
Storage Note
Store at 4°C, avoid freeze-thaw cycles.
Application Notes
Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20ul of 300ug/ml murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD3 antibody diluted to 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Hampster IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 33% sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to background.
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