CHAT Antibody (clone 1.B3.9B3)
Catalogue Number: LS-C35920-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.09% sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CHAT, Choline acetylase, Choline acetyltransferase, Choline O-acetyltransferase, CMS1A2, CHOACTASE, CMS1A |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 1.B3.9B3 |
| Isotype: | IgG1 |
| Immunogen: | Highly purified ChAT obtained from porcine brain |
| Application: | ELISA, WB, IHC, IHC-Pfa |
Additional Text
Concentration
0.5 mg/ml
Gene ID
1103
Purification
Purified
Gene Name
CHAT
Short Description
CHAT antibody LS-C35920 is an unconjugated mouse monoclonal antibody to CHAT from pig. It is reactive with human, rat and pig. Validated for ELISA, IHC and WB.
Storage Note
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
Fixation: a) Human tissue: Fix by placing fresh (5-8 h post mortem) tissue in a 1:1 solution of 4% paraformaldehyde/10% formaldehyde for 1 week at 4°C. Divide into tissue blocks (basal forebrain, septum and basal ganglia). Incubate the blocks in sucrose solutions of increasing concentration (10%, 20% and 30%). Prepare 50um sections and store in a cryoprotective solution at -15°C (3).b) Rat tissue: Brain and spinal cord marrow tissue (transcardial perfusion). Perform a transcardial perfusion initially with oxygen-saturated heparin solution (II) (100-200ml) and subsequently with paraformaldehyde/picric acid solution (III) (400ml). Carry out immediate tissue preparation and fix for a further 30 minutes in the same solution. Wash out the fixative with several fresh quantities of Buffer A. Prepare 50um sections.
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