AKT3 Antibody (aa119-131)

Catalogue Number: LS-C3627-LSP

Manufacturer:LifeSpan BioSciences Inc.
Preservative:0.05% Sodium azide
Type:Polyclonal Primary Antibody - Unconjugated
Alias:AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kinase Akt-3, RAC-gamma, MPPH, PKB-GAMMA, PKBG, Protein kinase B gamma, RAC-PK-gamma, STK-2
Shipping Condition:RT
Unit(s): 100 ul
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: KLH-conjugated, synthetic peptide corresponding to aa119-131 (CSPTSQIDNIGEE) of human Akt3/PKBg. The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster, Zebrafish (92%).
Application: IP, WB, FA

Additional Text

Gene Name

AKT3

Gene ID

10000

Purification

Affinity Purified

Antigen Type

Synthetic Peptide, KLH Conjugated

Short Description

AKT3 antibody LS-C3627 is an unconjugated rabbit polyclonal antibody to AKT3 (aa119-131) from human. It is reactive with human, mouse, rat and other species. Validated for Func, IP and WB.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

Suitable for use in Western Blot and Immunoprecipitation. Western Blot: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates. Immunoprecipitation: 5 ul immunoprecipitated active Akt3/PKBg from 15 nM IGF-I stimulated HEK293 cell lysates. The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg).