HES4 Antibody (aa94-221)

Catalogue Number: LS-C36653-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	HES4, BHLHb42, Transcription factor HES-4, BHLH factor Hes4, Hairy and enhancer of split 4, HHES4
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:	E. coli-derived rhHES-4 (aa 94-221).
Application:	ELISA, ICC, WB

Overview

Additional Text

Gene Name

HES4

Purification

Affinity Purified

Gene ID

57801

Short Description

HES4 antibody LS-C36653 is an unconjugated sheep polyclonal antibody to human HES4 (aa94-221). Validated for ELISA, ICC and WB.

Antibody Clonality

Polyclonal

Application Notes

Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.

Price

£1,157.00

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