ITGB5 / Integrin Beta 5 Antibody (Extracellular Domain)

Catalogue Number: LS-C36943-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	ITGB5, Integrin Beta 5, Integrin beta-5, Integrin beta-5 subunit, Integrin, beta 5
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:	CHO cell-derived rhIntegrin b5 extracellular domain.
Application:	ELISA, FC, ICC, WB

Overview

Additional Text

Gene ID

3693

Gene Name

ITGB5

Purification

Affinity Purified

Storage Note

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 0.1-0.2 ug/ml. Detection limit ~5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Immunocytochemistry: 10 ug/ml to detect b5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. Detection limit ~0.2 ng/well.

Short Description

Integrin Beta 5 antibody LS-C36943 is an unconjugated sheep polyclonal antibody to human Integrin Beta 5 (ITGB5) (Extracellular Domain). Validated for ELISA, Flow, ICC and WB. Cited in 2 publications.

Price

£1,197.00

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