KLK13 / Kallikrein 13 Antibody (aa19-262)

Catalogue Number: LS-C37112-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	KLK13, Kallikrein 13, Kallikrein-13, KLK-L4, KLKL4, Kallikrein-like protein 4, Kallikrein-like gene 4
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Goat
Clone:
Isotype:	IgG
Immunogen:	NS0-derived rhKLK13 (aa19-262).
Application:	ELISA, IP, WB, NT

Overview

Additional Text

Gene ID

26085

Purification

Affinity Purified

Gene Name

KLK13

Short Description

Kallikrein 13 antibody LS-C37112 is an unconjugated goat polyclonal antibody to human Kallikrein 13 (KLK13) (aa19-262). Validated for ELISA, IP, Neut and WB.

Antibody Clonality

Polyclonal

Application Notes

Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50% of the proteolytic activity was inhibited by the antibody at approximately 8.6 ug/ml (IC50) under conditions in which the enzyme was present at 1 ug/ml and the substrate concentration was 100 M. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well.

Price

£1,170.00

-

Quantity
Unit Size

Find out about delivery