SOX15 Antibody
Catalogue Number: LS-C39197-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Physical state: | Lyophilized |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SOX15, Protein SOX-15, Protein SOX-20, SOX26, SOX20, Protein SOX-12, SOX27 |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Sheep |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | E. coli-derived rhSOX15. |
| Application: | ELISA, ICC, WB |
Additional Text
Gene Name
SOX15
Purification
Affinity Purified
Gene ID
6665
Short Description
SOX15 antibody LS-C39197 is an unconjugated sheep polyclonal antibody to human SOX15. Validated for ELISA, ICC and WB.
Antibody Clonality
Polyclonal
Application Notes
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 1% cross-reactivity with rhSOX1, -2, -5, -6, -7, -10, -12, -14, -15, -17, and rhSOX21. Immunocytochemistry: This antibody can be used at a concentration of 10 ug/ml to detect SOX15 in NTera2 teratocarcinoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 0.3 ng/well.
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