HMG1 / HMGB1 Antibody

Catalogue Number: LS-C663094-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Shelf Life:	12 months
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	HMGB1, Amphoterin, High mobility group protein B1, High mobility group box 1, High mobility group protein 1, HMG1, HMG3, SBP-1, High-mobility group box 1, HMG-1
Shipping Condition:	RT
Storage Condition:	2-8°C
Unit(s):	100 ug
Host name:	Armenian Hamster
Clone:
Isotype:	IgG
Immunogen:
Application:	FC, IF, WB

Overview

Additional Text

Gene ID

3146

Gene Name

HMGB1

Short Description

HMGB1 antibody LS-C663094 is an unconjugated hamster-armenian monoclonal antibody to HMGB1 (HMG1) from rat. It is reactive with human, mouse and rat. Validated for Flow, IF and WB.

Antibody Clonality

Monoclonal

Storage Note

Store at 4°C for up to 1 year.

Purification

Filtered

Application Notes

FC: For staining cells were permeabilized with buffer containing 0.5% saponin. The cells were fixed in 0.01% formaldehyde before staining. Hamster IgG isotype control is used as a negative control. IF: NIH-3T3 cells were fixed with 2% PFA (15 min). After fixation, cells were washed with PBS and incubated for 5 min at 4C with permeabilization buffer . The cells were blocked with 1% BSA/10% goat serum in PBS for 10 min and were incubated with HMGB1 mAb 5H6 (10 ug/ml) overnight at 4C. The secondary antibody was biotinylated goat anti-hamster IgG at a 1:500 dilution for 1 hour. Finally, streptavidin was used at a 1:500 dilution for 30 min. W: A band is expected at ~30 kDa using reducing conditions. For non-reducing conditions a band is expected at both 27 kDa and 30 kDa.

Price

£642.00

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