FOXH1 Antibody (aa164-272)

Catalogue Number: LS-C66336-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	FOXH1, Fast-2, FAST2, FAST-1, FAST1, Forkhead box H1, Forkhead box protein H1, HFAST-1
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:	E. coli-derived recombinant human rhFoxH1 (aa 164 - 272).
Application:	ELISA, ICC, WB

Overview

Additional Text

Gene ID

8928

Gene Name

FOXH1

Antigen Type

Recombinant Protein

Purification

Affinity Purified

Short Description

FOXH1 antibody LS-C66336 is an unconjugated sheep polyclonal antibody to human FOXH1 (aa164-272). Validated for ELISA, ICC and WB.

Antibody Clonality

Polyclonal

Application Notes

Suitable for use in Western blot, Immunocytochemistry and Direct ELISA. Western blot: 0.1-0.2 ug/ml. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect human FoxH1 in human BG0IV stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at room temperature for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. The detection limit is ~0.2 ng/well. Hybridoma: Produced in sheep immunized with purified, E. coli-derived, recombinant human FoxH1. (rhFoxH1; aa 164-272; Accession # O75593).

Price

£1,157.00

-

Quantity
Unit Size

Find out about delivery