Catalogue Number: LS-C6821-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | FGF6, FGF-6, Fibroblast growth factor 6, HSTF-2, HBGF-6, HST-2, HST2, HSTF2 |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Human, recombinant Fibroblast Growth Factor 6 (FGF6) from E. coli. |
| Application: | ELISA, WB, FA, NT |
2251
FGF6
Recombinant Protein
Affinity Purified
FGF6 antibody LS-C6821 is an unconjugated goat polyclonal antibody to human FGF6. Validated for ELISA, Func, Neut and WB.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Polyclonal
Suitable for use in Neutralization, Western Blot and ELISA. Neutralization: ND50 is ~0.025-0.075 ug/ml in the presence of 1 ng/ml of rhFGF6 and 1 ug/ml of heparin, using the FGF6 responsive NR6R-3T3 fibroblasts as target cells. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is approximately 2 ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is ~0.16 ng/well.