NOTCH1 Antibody (aa2428-2556)

Catalogue Number: LS-C73690-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	NOTCH1, Notch 1, TAN1
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:	E. coli-derived recombinant human rhNotch-1 (aa 2428 - 2556).
Application:	ELISA, FC, ICC, WB

Overview

Additional Text

Gene ID

4851

Antigen Type

Recombinant Protein

Purification

Affinity Purified

Gene Name

NOTCH1

Short Description

NOTCH1 antibody LS-C73690 is an unconjugated sheep polyclonal antibody to human NOTCH1 (aa2428-2556). Validated for ELISA, Flow, ICC and WB.

Antibody Clonality

Polyclonal

Application Notes

Suitable for use in for use in Western blot, Immunocytochemistry, Flow cytometry. and Direct ELISA. Western blot: 0.1-0.2 ug/ml. The detection limit for rhNotch-1 is ~0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect human Notch-1 in SAOS human osteosarcoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for. 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Flow cytometry: 50 ug/ml and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10il of a 25 ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Notch-1. The detection limit is ~0.2 ng/well.

Price

£1,157.00

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