PD-L2 / PDCD1LG2 / CD273 Antibody

Catalogue Number: LS-C74220-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Goat
Clone:
Isotype:	IgG
Immunogen:	NS0-derived rmPD-L2.
Application:	ELISA, FC, WB, IHC, FA

Overview

Additional Text

Gene ID

80380

Gene Name

PDCD1LG2

Antigen Type

Recombinant Protein

Purification

Affinity Purified

Short Description

CD273 antibody LS-C74220 is an unconjugated goat polyclonal antibody to mouse CD273 (PD-L2 / PDCD1LG2). Validated for ELISA, Flow, Func, IHC and WB.

Antibody Clonality

Polyclonal

Application Notes

Western Blot, Immunohistochemistry, Flow Cytometry, Blocking of receptor-ligand interaction, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 ug/mL with the appropriate secondary reagents to detect mouse PD-L2. The detection limit for rmPD-L2 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-L2 in cells and tissues. The working dilution is 2-15 ug/mL. Flow Cytometry: This antibody has been tested on Tim-1 stimulated RAW 264.7 cells for use in flow cytometry. Dilute this antibody to 50 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Blocking of receptor-ligand interaction: Approximately 1-4 ug/mL of this antibody will block 50% of the binding of 1 ug/mL of rmPD-1 to immobilized rmPD-L2/Fc chimera (100 ul of a 1 ug/mL solution was coated in each well) in a functional ELISA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect mouse PD-L2. The detection limit for rmPD-L2 is approximately 1.2 ng/well.

Price

£1,157.00

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