COL1A1 / Collagen I Alpha 1 Antibody

Catalogue Number: LS-C744029-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	COL1A1, Alpha-1 type I collagen, Collagen alpha 1 chain type I, Collagen alpha-1(I) chain, Collagen, type I, alpha 1, Collagen I, Pro-alpha-1 collagen type 1, OI4
Shipping Condition:	RT
Unit(s):	0.5 ml, 0.1 ml
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	Purified collagen type I from bovine skin
Application:	ELISA, IF, IHC-P, WB, RIA

Overview

Additional Text

Concentration

0.5 mg/ml

Gene ID

1277

Gene Name

COL1A1

Short Description

Collagen I Alpha 1 antibody LS-C744029 is an unconjugated rabbit polyclonal antibody to Collagen I Alpha 1 (COL1A1) from bovine. It is reactive with human, bovine, cow and other species. Validated for ELISA, IF, IHC, RIA and WB.

Storage Note

Store at -20°C.

Antibody Clonality

Polyclonal

Application Notes

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type I 100%, bovine collagen II, IX, XI <0.1%, sheep collagen type I 50 % (IHC estimated), bovine fibronectin <0.1%, human collagen type I <2%; chicken collagen type I <0.1% (estimates except sheep collagen I from solid phase RIA at a 1:500 dilution ).

Price

£1,182.00

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