COL3A1 / Collagen III Antibody

Catalogue Number: LS-C744081-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	COL3A1, Alpha1 (III) collagen, Collagen alpha 1(iii), Collagen alpha-1(III) chain, Collagen, type III, alpha 1, Collagen, fetal, EDS4A
Shipping Condition:	RT
Unit(s):	0.1 ml
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	Procollagen type III deprived of the C-Terminus PIIICP peptide
Application:	ELISA, IF, IHC-P, WB, RIA

Overview

Additional Text

Gene Name

COL3A1

Concentration

0.5 mg/ml

Gene ID

1281

Short Description

Collagen III antibody LS-C744081 is an unconjugated rabbit polyclonal antibody to Collagen III (COL3A1) from human. It is reactive with human, cow and pig. Validated for ELISA, IF, IHC, RIA and WB.

Storage Note

Store at -20°C.

Antibody Clonality

Polyclonal

Application Notes

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Collagen type III is synthesized as a homo-trimeric pro-collagen comprising three identical pro-alpha(III)-chains. It has been reported that procollagen type III is processed extracellularly at the ECM and can be found by immunostaining intracellular as well as extracellular. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human and bovine procollagen type III (PIIIP) 100%; human and bovine procollagen type and collagen type I <0.1% in RIA at 1:200 dilution.

Price

£602.00

-

Quantity
Unit Size

Find out about delivery