ELN / Elastin Antibody

Catalogue Number: LS-C744107-LSP

Manufacturer:LifeSpan BioSciences Inc.
Type:Polyclonal Primary Antibody - Unconjugated
Alias:ELN, SVAS, WBS, Elastin, Tropoelastin, WS
Shipping Condition:RT
Unit(s): 0.5 ml
Host name: Rabbit
Clone:
Isotype:
Immunogen: Purified elastin from rat aorta
Application: ELISA, IF, IHC-P, RIA

Additional Text

Concentration

0.5 mg/ml

Gene ID

2006

Gene Name

ELN

Short Description

Elastin antibody LS-C744107 is an unconjugated rabbit polyclonal antibody to Elastin (ELN) from rat. It is reactive with mouse and rat. Validated for ELISA, IF, IHC and RIA.

Storage Note

Store at -20°C.

Antibody Clonality

Polyclonal

Application Notes

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties. Rat elastin 100%, rat collagen type I, II, III and V <0.1% ( RIA at 1:500 dilution).