Catalogue Number: LS-C744107-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ELN, SVAS, WBS, Elastin, Tropoelastin, WS |
| Shipping Condition: | RT |
| Unit(s): | 0.5 ml |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Purified elastin from rat aorta |
| Application: | ELISA, IF, IHC-P, RIA |
0.5 mg/ml
2006
ELN
Elastin antibody LS-C744107 is an unconjugated rabbit polyclonal antibody to Elastin (ELN) from rat. It is reactive with mouse and rat. Validated for ELISA, IF, IHC and RIA.
Store at -20°C.
Polyclonal
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties. Rat elastin 100%, rat collagen type I, II, III and V <0.1% ( RIA at 1:500 dilution).