Mouse Anti-Human IgG4 Fc Secondary Antibody (Fc, HRP)
Catalogue Number: LS-C744218-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Type: | Monoclonal Secondary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 0.2 mg |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | ELISA, ICC, IHC-P, WB, DB |
Additional Text
Concentration
0.5 mg/ml
Short Description
Human IgG4 Fc antibody LS-C744218 is an HRP-conjugated mouse monoclonal antibody. Validated for DB, ELISA, ICC, IHC and WB.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
The reactivity of the antiserum is restricted to the subclass specific determinant on the pFc portion of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
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