Beta Amyloid 1-42 Antibody (clone Ab42.2)
Catalogue Number: LS-C759916-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | Ab42.2 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to Aβ35-42. |
| Application: | ELISA, IF, IP, sELISA, WB, IHC |
Additional Text
Concentration
0.5 mg/ml
Antigen Type
Synthetic Peptide
Purification
Protein G purified
Short Description
Beta Amyloid 1-42 antibody LS-C759916 is an unconjugated mouse monoclonal antibody to Beta Amyloid 1-42 from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC, IP, SwELISA and WB.
Storage Note
Store at -20°C for up to 1 year.
Antibody Clonality
Monoclonal
Application Notes
Immunoblotting, Immunohistochemistry: Immunofluorescence, Immunoprecipitation Test at 1-10ug/ml. Sandwich ELISA (as capture antibody with #57004 as HRP-conjugated detection antibody). These are recommended concentrations; enduser should determine optimal concentrations for their applications. 96-well ELISA plates are coated with capture MAb at 2.5-5ug/well and incubated at 4oC overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4 oC overnight. The next day plates are washed in PBS and serial dilution of Aβ samples are added; plates are incubated overnight at 4oC. The next day plates are washed in PBS, and HRP-conjugated detection Aβ MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins.
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