Beta Amyloid 1-40 Antibody (clone Ab40.1)
Catalogue Number: LS-C759920-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | Ab40.1 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to Aβ35-40. |
| Application: | ELISA, IF, IP, sELISA, WB, IHC |
Additional Text
Antigen Type
Synthetic Peptide
Purification
Protein G purified
Storage Note
Store at -20°C for up to 1 year.
Antibody Clonality
Monoclonal
Short Description
Beta Amyloid 1-40 antibody LS-C759920 is an unconjugated mouse monoclonal antibody to Beta Amyloid 1-40 from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC, IP, SwELISA and WB.
Concentration
1 mg/ml
Application Notes
Immunoblotting, Immunohistochemistry: Immunofluorescence, Immunoprecipitation, Test at 1-10ug/ml. Sandwich ELISA (works equally well as capture or detection antibody). These are recommended concentrations; enduser should determine optimal concentrations for their applications. 96-well ELISA plates (Immulon HBX4) are coated with capture MAb at 2.5-5ug/well and incubated at 4oC overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4oC overnight. The next day plates are washed in PBS and serial dilution of Aβ samples are added; plates are incubated overnight at 4oC. The next day plates are washed in PBS, and HRP-conjugated detection Aβ MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins.
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