Catalogue Number: LS-C76211-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | TAG-72 |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG1, kappa |
| Immunogen: | Membrane-enriched fraction of a human breast carcinoma liver metastasis and purified TAG-72 protein. MW: >1000kD. |
| Application: | FC, IHC-P, IP, WB, IHC |
Purified protein
Protein G purified
TAG-72 antibody LS-C76211 is an HRP-conjugated mouse monoclonal antibody to TAG-72 from human. It is reactive with human and rat. Validated for Flow, IHC, IP and WB.
Monoclonal
Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation, Radioimaging and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2hrs at RT. Immunoprecipitation: Use Protein G, 2 ug/mg protein lysate. Immunohistochemistry, Formalin-fixed, paraffin-embedded: 0.2-0.4 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Ls174T cells. Colon Carcinoma. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.