CCR7 Antibody (clone 4B12)
Catalogue Number: LS-C78053-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Physical state: | Lyophilized |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CCR7, BLR2, Chemokine (C-C) receptor 7, CD197, C-C CKR-7, CMKBR7, CC chemokine receptor 7, CCR-7, CD197 antigen, CDw197, EBI1, EVI1, MIP-3 beta receptor, C-c chemokine receptor 7, C-C chemokine receptor type 7, CC-CKR-7, Chemokine c-c motif receptor 7 |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Rat |
| Clone: | 4B12 |
| Isotype: | IgG2a |
| Immunogen: | RBC-2H3 cells expressing mCCR7. |
| Application: | FC, IHC, FA, NT |
Additional Text
Gene ID
1236
Antigen Type
Cells
Purification
Protein G purified
Gene Name
CCR7
Short Description
CCR7 antibody LS-C78053 is an unconjugated rat monoclonal antibody to mouse CCR7. Validated for Flow, Func, IHC and Neut.
Storage Note
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Application Notes
Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 ug/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 ug/mL in the presence of 25 ng/mL of rmMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.
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