IL13 Antibody (Azide-free)
Catalogue Number: LS-C8144-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | IL13, ALRH, BHR1, Interleukin-13, NC30, p600, IL-13, Interleukin 13 |
| Shipping Condition: | RT |
| Unit(s): | 500 ug |
| Host name: | Rat |
| Clone: | |
| Isotype: | IgG1, kappa |
| Immunogen: | COS-expressed recombinant human IL-13. |
| Application: | ELISA, FC, IF, sELISA, WB, NT |
Additional Text
Gene ID
3596
Antigen Type
Recombinant Protein
Purification
Purified
Gene Name
IL13
Short Description
IL13 antibody LS-C8144 is an unconjugated rat monoclonal antibody to human IL13. Validated for ELISA, Flow, IF, Neut, SwELISA and WB.
Antibody Clonality
Monoclonal
Storage Note
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Application Notes
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control. Western Blot: Useful for Western blot applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
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