VEGFA / VEGF Antibody (clone R010, Preservative Free)
Catalogue Number: LS-C819200-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1 |
| Shipping Condition: | RT |
| Unit(s): | 200 ug, 500 ug |
| Host name: | Rabbit |
| Clone: | R010 |
| Isotype: | IgG |
| Immunogen: | Recombinant Human VEGF165/VEGFA Protein |
| Application: | ELISA, NT, Blk |
Additional Text
Gene ID
7422
Antigen Type
Recombinant Protein
Purification
Protein A purified
Gene Name
VEGFA
Concentration
1.85 mg/ml
Short Description
VEGF antibody LS-C819200 is an unconjugated rabbit monoclonal antibody to human VEGF (VEGFA). Validated for Block, ELISA and Neut.
Antibody Clonality
Monoclonal
Storage Note
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Application Notes
Block: In a functional ELISA which immobilized recombinant Human VEGF165 at 1 ug/ml (100 µL/well) in the plate, the Rabbit anti-Human VEGF165 Monoclonal Antibody can block the binding of 5 ug/ml of biotinylated recombinant Human VEGFR2 to human VEGF165, the EC50 is 1. 51 ug/ml. Neutralization: The neutralization activity of VEGF Neutralizing Antibody is measured by its ability to neutralize VEGF165 induced proliferation in HUVEC human umbilical vein endothelial cells. The VEGF Neutralization titer (IC50) is typically 5. 35-21. 4 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165. Please Note: Optimal concentrations/dilutions should be determined by the end user.
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