TNF Alpha Antibody (clone R101, Preservative Free)

Catalogue Number: LS-C820676-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Shelf Life:	12 months
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
Shipping Condition:	RT
Unit(s):	200 ug, 500 ug
Host name:	Rabbit
Clone:	R101
Isotype:	IgG
Immunogen:	Recombinant Human TNF-alpha / TNFA Protein
Application:	FC, NT, Blk

Overview

Additional Text

Gene ID

7124

Antigen Type

Recombinant Protein

Purification

Protein A purified

Gene Name

TNF

Short Description

TNF Alpha antibody LS-C820676 is an unconjugated rabbit monoclonal antibody to human TNF Alpha. Validated for Block, Flow and Neut.

Antibody Clonality

Monoclonal

Storage Note

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Application Notes

Block: In a functional ELISA which immobilized recombinant Human TNFR1 at 1ug/ml (100 µL/well) in the plate, the Rabbit Anti-Human TNFa Monoclonal Antibody can block the binding of 0.2 ug/ml of biotinylated Human TNFa to human TNFR1, the EC50 is 1. 25 ug/ml;In a functional ELISA which immobilized recombinant Human TNFR2 at 2 ug/ml (100 µL/well) in the plate, the Rabbit Anti-Human TNFa Monoclonal Antibody can block the binding of 0.2 ug/ml of biotinylated Human TNFa to human TNFR1, the EC50 is 1. 26 ug/ml. Neutralization: The neutralization activity of TNFa Neutralizing Antibody is Measured by its ability to neutralize TNF-a induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 8. 8-35. 2 ng/mL in the presence of 2 ng/mL Recombinant Human TNF-a and 1 ug/ml actinomycin D. Please Note: Optimal concentrations/dilutions should be determined by the end user.

Concentration

3.69 ug/ul

Price

£311.00

-

Quantity
Unit Size

Find out about delivery