RAF1 / RAF Antibody (aa657-668)

Catalogue Number: LS-C9887-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Preservative:	0.05% Sodium azide
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:	12 residue synthetic C-Terminus peptide (CTLTTSPRLPVF) corresponding to aa 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).
Application:	IP, WB

Overview

Additional Text

Gene ID

5894

Antigen Type

Synthetic Peptide

Purification

Protein A purified

Gene Name

RAF1

Short Description

RAF antibody LS-C9887 is an unconjugated sheep polyclonal antibody to RAF (RAF1) (aa657-668) from human. It is reactive with human, mouse, rat and other species. Validated for IP and WB.

Storage Note

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 ug/ml detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 ug immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro.

Price

£705.00

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