Human SEMA3A / Semaphorin 3A Protein (Recombinant His, N-terminus + Human IgG Fc, C-terminus)
Catalogue Number: LS-G133792-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Molecular Weight: | 226.2 kDa or 200 kDa or 120-130 kDa, 90-100 kDa, and 35-40 kDa |
| Physical state: | Lyophilized |
| Type: | Fusion Protein |
| Alias: | SEMA3A, Collapsin 1, Coll-1, Hsema-III, Hsema-I, Sema III, SEMAIII, SEMAL, Semaphorin III, Semaphorin-3A, Semaphorin-iii (hsema-i), SemD, SEMA1, Semaphorin D, COLL1, SEMAD, Semaphorin 3A, Semaphorin L, SEM3A |
| Host Cell: | CHO Cells |
| Shipping Condition: | RT |
| Unit(s): | 25 ug, 5 ug |
Description
Description: Semaphorins are a large group of structurally-related, secreted, GPI-anchored, transmembrane, cell-signaling molecules. There are 8 major classifications of Semaphorins (the first seven ordered by number, 1-7, and the eighth designated V for virus), which are characterized by the existence of a conserved 500 amino acid SEMA domain at the amino terminus. Classes 3, 4, 6, and 7 are found in vertebrates only, whilst class 5 is found in both vertebrates and invertebrates. Each class is then divided into additional subgroups based on shared structural characteristics. Semaphorins primarily function as axon growth cone guidance factors during neuronal development. Semaphorin 3A acts as a chemo-repellent to axons, and an inhibitor of the growth of axons by signaling through receptors, Neuropilin-1 and Plexin-A. PeproTech's CHO cell-derived Recombinant Human Semaphorin 3A Fc is a glycosylated, disulfide-linked homodimer of 1,976 amino acid residues, which includes the SEMA domain, immunoglobulin c2-like domain, and the C-terminal basic Arg/Lys-rich domain of the mature sequence, as well as an 8-residue N-terminal His-tag and a 230-residue C-terminal Fc region linked by two glycines. Recombinant Human Semaphorin 3A Fc has a calculated molecular weight of 226.2 kDa and therefore runs above the 200kDa marker by SDS-PAGE analysis under nonreducing conditions. When run under reducing conditions, this protein migrates as three distinct bands that, due to glycosylation, run higher than expected at apparent molecular weights of approximately 120-130 kDa, 90-100 kDa, and 35-40 kDa.
Additional Text
Gene ID
10371
Gene Name
SEMA3A
Protein Tag
hIgG Fc Tag, HIS Tag
Storage Note
Store at -20°C. Protect from moisture.
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations