Human Skeletal Muscle Protein (Cell/Tissue Lysate)

Catalogue Number: LS-G134148-LSP

Manufacturer:LifeSpan BioSciences Inc.
Shelf Life:6 months
Type:Tissue Lysate
Host Cell:human
Shipping Condition:Blue Ice
Unit(s): 0.1 mg

Description

Description: Heat inactivated Skeletal Muscle Lysate sourced from Human Skeletal Muscle Tissue product is a tissue lysate which was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.

Additional Text

Concentration

2 mg/ml

Storage Note

Short term: Store at 2-8°C. Long term: Aliquot and freeze at -70°C for up to 6 months. Avoid freeze-thaw cycles.