Catalogue Number: LS-G97104-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Molecular Weight: | 158 kDa |
| Type: | Other Recombinant Protein |
| Alias: | PRKAA2, ACACA kinase, Alpha2 AMPK, AMPK alpha 2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, AMPK-2, AMPKa2, AMPKalpha2, Ampk alpha2, AMPK2, PRKAA, AMPK, AMPK-alpha-2 chain, HMGCR kinase |
| Host Cell: | Baculovirus |
| Shipping Condition: | Blue Ice |
| Storage Condition: | -70°C |
| Unit(s): | 50 ug |
Description: The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 40, 42 & 95 KDa respectively in SDS-PAGE under reducing conditions. A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
PRKAA2
5563
Store at -70°C.