Human Ku70+Ku80 / XRCC6+XRCC5 Protein (Recombinant) (Met1-Ile732)
Catalogue Number: LS-G97107-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Molecular Weight: | 157 kDa |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Host Cell: | Baculovirus |
| Shipping Condition: | Blue Ice |
| Storage Condition: | -70°C |
| Unit(s): | 50 ug |
Description
Description: The recombinant heterodimer of human XRCC5/XRCC5 comprises 1379 (751 + 628) amino acids and has a calculated molecular mass of 157 (85 + 72) kDa. The apparent molecular mass of rh XRCC5/XRCC5 heterodimer is approximately 70 & 85 kDa respectively in SDS-PAGE under reducing conditions. A DNA sequence encoding the XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Additional Text
Storage Note
Store at -70°C.
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